washing twice and re-suspending in PBS. Volume (0.1 ml) of each suspension containing approximately 107 cells/ml, as determined by a haemocytometer slide at a magnification of 400 on a Leica semi-motorized research microscope (Model DM3000; Germany),
washing twice and re-suspending in PBS.Volume (0.1 ml) of each suspension containing approximately107 cells/ml, as determined by a haemocytometer slide at amagnification of 400 on a Leica semi-motorized research microscope(Model DM3000; Germany),