In plant-pathogen interactions, though ISR by PGPR is well documented (van Loon et al., 1998 and Kloepper et al., 2004), little is known about the ability of a strain mixture to reduce disease incidence both locally and systemically through induction of host defence enzymes. The present study therefore assessed the efficacy of in vitro selected rhizobacterial mixtures along with individual strains on vascular wilt reduction in tomato in both localized and induced systemic resistance experiments under greenhouse conditions. The role of defence enzymes in disease suppression by the biocontrol agents was also ascertained.