Extraction
The powdered sample was extracted with 95% ethanol by using cold method extraction in room temperaturefor one
week. The 95% ethanol extract was filtered, distilled and concentrated to obtain the solid greenish residue. The
95% ethanol extract was further partitioned successively with petroleum ether, n-hexane, chloroform, ethyl acetate,
ethanol, n-butanol and methanol. The solvents were recovered under reduced pressure.