The mixture was left to stand for 5 min at room temperature and then 2.5 mL of NED reagent was added with mixing, followed by dilution to volume. After 15 min, the absorbance was measured at 540 nm.
The mixture was left to stand for 5 min at room temperature and then 2.5 mL of NED reagent was added with mixing, followed by dilution to volume. After 15 min, the absorbance was measured at 540 nm.