After overlaying with soft TSA (0.60% of
agar-agar) inoculated with the indicator microorganism,
the tubules were removed and the obtained
wells were filled with 20 µl of thyme essential oil.
Plates are incubated at 37 °C during 24 h. The antimicrobial
activity of thyme, lactic acid (20 µl), and of combined
thyme oil and lactic acid (the compound included
50 ppm of lactic acid for 20 µl of essential oil) was
investigated in order to determine their synergistic
effect. Antibiogram tablets of clindamycin and nystatine
(30 mg) were used as a positive control for the
comparation of the antibacterial activity of essential
oils