The mixture was then thoroughly vortexed and the absorbance of the pink colour was measured at 510 nm against the blank. For calibration curve (+)-Catechin was used with a concentration range of 10–1000 lg/ml (Absorbance = 2.9337 catechin lg +0.034, R2 =0.99). Results were expressed as mg (+)-catechin equivalent (CE)/g of extract.