Fungal isolates
Metarhizium robertsii (ARSEF 2575) and B. bassiana (ARSEF 252)
were obtained from the United States Department of Agricultural
Research Service Collection of Entomopathogenic
Fungal Cultures, Ithaca, New York. These fungi were transformed
for green fluorescent protein (GFP) expression as previously
described (Fang et al. 2006). Cultures were grown on
potato dextrose agar (PDA; Difco Laboratories, BD, Sparks, MD,
USA) at 27 C for 10 d to obtain conidia