Histology
At the time of dissection, the weights of the body, the ovary
and the liver were recorded to calculate the gonadosomatic
index (GSI; 100 weight of the ovary/body weight) and hepatosomatic
index (HSI; 100 weight of the liver/body weight).
The ovaries were fixed in Bouin’s fluid for 24 h, dehydrated
through alcoholic grades, cleared in chloroform and embedded
in paraffin. Serial sections of 5 lm thickness were cut and
stained with hematoxylin–eosin.