Inoculation method (Agar well diffusion) Each strain was poured in two petri plates. One plate inoculated with each of the strains with oil. Other kept as control without oil. Inoculation of clove oil was done in three wells 50µl, 75µl and 100µl, in same plate, with the help of micropippete and punching tube in Laminar hood.
All the bacterial plates were incubated at 37 ˚Cfor 24 hours and fungal plates at 28° C for 3 days.