The weight-average diameters (d43) of the coconut milk emulsion
droplets and their size distribution (volume fraction as a function
of particle size) were measured using a laser diffraction particle
analyzer (Horiba, LA-920, Irvine, Calif., U.S.A.) assuming a relative
refractive index of 1.15. Coconut milk samples were diluted to approximately
0.001% fat before analysis to minimize multiple scattering
effects. In some experiments, the coconut milk was diluted
in 1 wt% sodium dodecyl sulfate (SDS, Sigma Chemical) solution
rather than water. SDS is an anionic surfactant that effectively displaces
protein from the surface of emulsion droplets and disrupts
droplet flocs formed due to interdroplet protein-protein interactions.
Particle size measured in water is referred to in this work as an
“effective” particle size and includes the presence of flocs, whereas
measurement in SDS solution is called the “primary” particle
size. Emulsion coalescence will be seen as a change in both primary
and effective particle diameter, whereas flocculation will increase
the effective diameter but the primary diameter will remain unchanged.
The weight-average diameters (d43) of the coconut milk emulsion
droplets and their size distribution (volume fraction as a function
of particle size) were measured using a laser diffraction particle
analyzer (Horiba, LA-920, Irvine, Calif., U.S.A.) assuming a relative
refractive index of 1.15. Coconut milk samples were diluted to approximately
0.001% fat before analysis to minimize multiple scattering
effects. In some experiments, the coconut milk was diluted
in 1 wt% sodium dodecyl sulfate (SDS, Sigma Chemical) solution
rather than water. SDS is an anionic surfactant that effectively displaces
protein from the surface of emulsion droplets and disrupts
droplet flocs formed due to interdroplet protein-protein interactions.
Particle size measured in water is referred to in this work as an
“effective” particle size and includes the presence of flocs, whereas
measurement in SDS solution is called the “primary” particle
size. Emulsion coalescence will be seen as a change in both primary
and effective particle diameter, whereas flocculation will increase
the effective diameter but the primary diameter will remain unchanged.
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