Saccharomyces cerevisiae efficiently carried out the fermentation of papaya juice for wine production. Total soluble solids (TSS) concentration, process temperature, Pectinase, inoculum level, pH were most significant factors affecting the ethanol production and their optimal values obtained 24ºBrix, 26ºC temperature, 5ml of pectinase enzyme, 10% inoculum, and pH 4.5. The optimum process conditions were experimentally verified. The present studies have revealed that papaya fruit could be successfully utilized for the production of wine. The high quality wines should have a characteristic bouquet and taste which depends on the cultivar, maturity and phytosanitary conditions of the papaya fruit, pedoclimatic conditions and most importantly, on yeast fermentation physiology. All these factors cause the different aroma, fragrance, composition and quality among all kinds of fruits wines.