Transportation of health care waste has scientifically evolved over the years. In a multi-centric analysis by IPEN study group.health care workers due to anatomical waste handling during segregation like human body parts, etc. health care waste should be transported in such a manner that it does not cause any stress . the trolleys were uncovered or overloaded Waste should never be transported directly by hand due to risk of accidental injury from infectious material or incorrectly disposed sharps.PPE were not worn by waste handlers in most of the cases possibly due to lack of understanding of its importance and climatic variations.