Prepare chopped liver broth (or cooked meat medium) for inoculation by heating 10 min
in boiling water or flowing steam and cooling rapidly without agitation. Inoculate 3 or 4
broth tubes with 2 ml of 1:10 homogenate as back-up for preceding plating procedure.
Incubate these tubes 24-48 h at 35°C in standard incubator. Disregard if plate counts for
viable C. perfringens are positive