Previous studies have demonstrated that lipid content in some microalgae increases during different
cultivation conditions such as nitrogen deprivation (Illman et al. 2000; Hsieh and Wu 2009), high light
intensity (Khotimchenko and Yakovleva 2005), and salt concentration (Araujo et al. 2011). The present
study was focused on the cultivation of two robust green algae strains Chlorella sp. and Scenedesmus sp.
extracted from Lithuanian lakes. The main objectives of the work were to investigate algae biomass growth
under different nitrogen (N) sources and concentrations, various concentrations of CO2 and
salinity with the aim to obtain optimum conditions for biomass cultivation for biofuel production.