The reducing power of the extract, which may serve as a
reflection of its antioxidant activity, was determined using a
modified Fe3þ to Fe2þ reduction assay, whereby the yellow
color of the test solution changes to various shades of green
and blue, depending on the reducing power of the sample. The
presence of antioxidants in the sample causes the reduction
of Fe3þ/ferricyanide complex to the Fe2þ form, which is
monitored by measuring the formation of Perl’s Prussian blue
at 700 nm [24]. In Fig. 2, all extracts show some degrees of
electron-donating capacity in a concentration-dependent
manner. The 100% ethanol extract once again gave the highest
reducing power and is significantly higher (p < 0.05) than
that of the other extracts at all concentrations studied, followed
by that of the 100% acetone extract, the 100% methanol
extract, and the extracts obtained by different concentrations
of aqueous acetone, aqueous ethanol, and aqueous methanol.
The reducing power of the 100% acetone extract at concentrations
ranging from 60 mg/mL to 100 mg/mL is insignificantly
higher than that of the 100% methanol extract and the extracts
of aqueous ethanol, aqueous methanol, and aqueous acetone.
The reducing power of the extract, which may serve as a
reflection of its antioxidant activity, was determined using a
modified Fe3þ to Fe2þ reduction assay, whereby the yellow
color of the test solution changes to various shades of green
and blue, depending on the reducing power of the sample. The
presence of antioxidants in the sample causes the reduction
of Fe3þ/ferricyanide complex to the Fe2þ form, which is
monitored by measuring the formation of Perl’s Prussian blue
at 700 nm [24]. In Fig. 2, all extracts show some degrees of
electron-donating capacity in a concentration-dependent
manner. The 100% ethanol extract once again gave the highest
reducing power and is significantly higher (p < 0.05) than
that of the other extracts at all concentrations studied, followed
by that of the 100% acetone extract, the 100% methanol
extract, and the extracts obtained by different concentrations
of aqueous acetone, aqueous ethanol, and aqueous methanol.
The reducing power of the 100% acetone extract at concentrations
ranging from 60 mg/mL to 100 mg/mL is insignificantly
higher than that of the 100% methanol extract and the extracts
of aqueous ethanol, aqueous methanol, and aqueous acetone.
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