2.7. Preparation of inoculated tea samples for HPLC analysis
Green and black tea infusions were used for the experiments. Aqueous extracts were made by adding 2 g of tea leaves to
100 ml of boiling water and allowing the suspension to stand for 10 min. The infusion was then filtered through cotton to eliminate rough particles, and then sterile filtered through a 0.45-lm membrane. The infusions obtained were approximately the strength of a ‘‘normal cup of tea”