Different lac dye concentrations were freshly prepared in
deionized water. The pH of the dye solution was adjusted to
3.0 with glacial acetic acid. The experiments were carried
out by shaking pretreated or untreated cotton with a 0.3%
(v/v) aqueous solution of chitosan (0.5 g) with different concentrations
of dye solution (50 mL) in a conical flask
(125 mL) at 10, 30 and 60 C in a thermostatted shaker bath
operated at 75 rpm.