Fresh pineapples (Ananas comosus cv. Josapine) were purchased from Pasar Borong Selangor, Malaysia. Pineapple fruit of regular shape and uniform size without any defect were selected. Fruit at maturity stage 5 (about 50% of eyes were orange-yellow, half ripe fruit) were used. The stage of maturity was determined based on the Malaysian standard by Federal Agricultural Marketing Authority (FAMA) (Shamsudin et al., 2009).