Isolation
Samples were transported to the lab under sterile conditions and one gram of the sample was transferred in a 250 mL flask containing sterile double distilled water (ddH2O), which was made up to 100 mL. The mixture in the flask was shaken for 15 - 20 min (150 rpm at 37˚C) for getting detached the attached surface microflora. One mL of this sample was plated after sufficient serial dilu-tions (up to 10−7) on Mullen Hinton Agar (MHA) plates and incubated for 24 - 48 h at 37˚C.