The oysters used in the present study were produced in 2012 at the
Ifremer experimental hatchery in La Tremblade (Charente, France). To
produce diploid and triploid oysters, 30 diploid females, 10 diploid
males and 15 tetraploidmaleswere selected as genitors. Diploid oysters
were three-year-old individuals, bought from local oyster farmers
working exclusively with wild-caught spat. These diploid oysters were
conditioned to full maturation in an open circulating system at
20–22 °C. Tetraploid oysters used to produce triploid progeny in
this work, were two years old and were conditioned to maturation in
the same conditions as the diploids, but in a quarantine area equipped
with water treatment systems using ozone to prevent dissemination of
gametes or larvae. Tetraploid oysters were used as males and sampled
from the same tetraploid broodstock used for the mass production of
triploids by French commercial hatcheries. This tetraploid broodstock
was initially produced in 2006 in the Ifremer experimental hatchery
using diploid × diploid crosses according to the method developed by
Benabdelmouna and Ledu (2007).
The oysters used in the present study were produced in 2012 at theIfremer experimental hatchery in La Tremblade (Charente, France). Toproduce diploid and triploid oysters, 30 diploid females, 10 diploidmales and 15 tetraploidmaleswere selected as genitors. Diploid oysterswere three-year-old individuals, bought from local oyster farmersworking exclusively with wild-caught spat. These diploid oysters wereconditioned to full maturation in an open circulating system at20–22 °C. Tetraploid oysters used to produce triploid progeny inthis work, were two years old and were conditioned to maturation inthe same conditions as the diploids, but in a quarantine area equippedwith water treatment systems using ozone to prevent dissemination ofgametes or larvae. Tetraploid oysters were used as males and sampledfrom the same tetraploid broodstock used for the mass production oftriploids by French commercial hatcheries. This tetraploid broodstockwas initially produced in 2006 in the Ifremer experimental hatcheryusing diploid × diploid crosses according to the method developed byBenabdelmouna and Ledu (2007).
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