It is very important to measure samples with different concentrations
since only then weak ECD signals can be observed (high
concentration), and at the same time appropriately low noise levels
are obtained (low concentration) for strong ECD signals. Two concentrations
were measured in methanol and are depicted in Fig. 5A
and B (UV: Fig. S13 A and B) to illustrate the concentration effects.
We want to emphasize that this is not related to concentration
effects due to aggregation. It is an important technical requirement.
An ECD spectrum measured in ethanol is also presented (Fig. 5C;
UV spectrum: Fig. S13C).