Methods
Tissue Culture
Young leaves and stem pieces were used for initiation of cell cultures. The explants were kept under running tap water for 30min. and then washed with Tween 20 (10% v/v) for 2-3 minutes. The residual of surfactant solution was removed by thorough washing with distilled water. These leaves were then subjected to surface sterilization using various surface sterilizing agents as shown in Table 1.