Coupons treated with sanitizers were transferred to centrifuge tubes (50 ml) containing 30 ml of Dey–Engley (DE) neutralizing broth (BBL/ Difco) and 3 g of glass beads to neutralize sanitizers, and vortexed for 1min atmaximumspeed
Coupons treated with sanitizers were transferred to centrifuge tubes(50 ml) containing 30 ml of Dey–Engley (DE) neutralizing broth (BBL/Difco) and 3 g of glass beads to neutralize sanitizers, and vortexed for1min atmaximumspeed