Inoculum preparation
The yeast isolates were streaked on YME medium with agar
(Wickerman, 1951), while the bacterial isolates were streaked on a
nutrient agar slants and all cultures were incubated for 24 h at
30°C. A loop of each culture of C. guilliermondii and B. subtilis was
inoculated in 50 ml of sterile inoculum medium (composed of the
stock medium without agar), then incubated at 30°C, 120 rpm for 24
h.