Among these samples, the crystallinity of GP-LRS3 was the greatest observed by X-ray diffraction. GP-LRS3 exhibited the highest the degree of ordered structure and the amorphous region of MPLRS3 was biggest by FT-IR and 13C NMR as well as UP-LRS3 displayed the highest degree of double helical structure. These results indicated that the structure of LRS3 was influenced by the preparation
methods. In addition, MP-LRS3 displayed the greatest solubility and swelling power, which were affected by the amorphous region, particle structure.