Two liters of autoclaved CB were inoculated with 2.0 mL of culture of each microbe separately and subjected to fermen-tation for 48 h. L. acidophilus and B. subtilis were incubated at 37◦C at pH 7.0, whereas S. cerevisiae was incubated at 32◦C atpH 4.0. The microbes grown on CB were directly sprayed on corn–soybean meal (1:1) followed by drying at 40◦C for 72 h.