In relation to the importance of phytochemicals for functional aspects of pepper, the capsaicinoids are a specific class
of compounds that causes the spicy sensation (pungency) of chili pepper fruit. The main capsaicinoid is capsaicin, followed
by dihydrocapsaicin, nordihydrocapsaicin, homodihydrocapsaicin, and homocapsaicin. Of these compounds, capsaicin
and dihydrocapsaicin account for approximately 90% of capsaicinoids in chili pepper fruit. These compounds are
the two most potent capsaicinoids; theirmolecules differ only in the saturation of an acyl group [7] and their amount represents a quality parameter for different kind of products. In addition, capsaicin has been used traditionally for muscular pain and headache and to improve circulation and also commonly added to herbal formulations because it acts as a catalyst for other herbs and aids in their absorption. Moreover, capsaicin and other capsaicinoids have shown strong evidence that have promising potential in the fight against many types of cancer [8].High concentrations of capsaicin occur in Chiltepin (C. annuum L. var. glabriusculum(Dunal)Heiser & Pickersgill) [9], Bhut Jolokia (C. chinense Jacq.) [10]; and the amounts vary with genotype, fruit maturity, and conditions of cultivation [11, 12].