Same volume of chloroform/isoamyl alcohol (24:1) was
added to the mixture and centrifuged at 12,000 g for 5 min afterwards
to precipitate out polysaccharides. The supernatant was
collected and equal volume of phenol/chloroform/isoamyl alcohol
(25:24:1) was added to it. The solution was thoroughly mixed and
centrifuged at 12,000 g, the proteins were precipitated out and
supernatant was collected.