In each treatment, 10 g flesh from 10 fruit was collected and
homogenized in 25 mL of ice-cold extraction buffer and 0.5 g
polyvinyl polypyrrolidone (PVPP) with a Kinematica tissue grinder
(Crl-6010, Kriens-LU, Switzerland). For CAT and SOD assays, the
extraction buffer was 50 mM sodium phosphate (pH 7.8). For
POD, 100 mM sodium phosphate buffer (pH 6.4) was used. The
homogenate was centrifuged at 27,000 ×g for 50 min at 4
◦
C and
the resulting supernatants were used directly for assay