Conclusions
Concentration of carbon and nitrogen source was main effect on lipid accumulation by oleaginous
yeast. In case of T. asahii, it could assimilate molasses and (NH4)2SO4 for lipid production which an optimal level of molasses and (NH4)2SO4 were 12% and 0.5 g/l, respectively. These indicating that it is possible to produce single cell oil by T. asahii for use as unsaturated fatty acid source in dairy cow diet.
Acknowledgments
Authors acknowledge the Thailand Research Fund through the Royal Golden Jubilee Ph.D. program for financial support. The center of Excellence
and Research Development office, Office of Higher Education Commission, Ministry of Education
(AG – BIO/PERDO – CE) and Agricultural Biotechnology Research Center for Sustainable Economy, Khon Kaen University for partially support. Department of Animal Science, faculty of Agriculture, Khon Kaen University and the Thermo-tolerant Dairy Cattle Research Group of Khon Kaen University for research facility support