For growth rate and enzymatic activity analysis, a single batch of larvae was used for
the different diet treatments. Because the time needed to obtain enough number of
oxygen consumption and ammonia excretion data, different batches of larvae maintained
in the same environmental conditions, were used for oxygen consumption and ammonia
excretion measurements in each diet treatment.
From M to PL stages, samples of ten shrimp were collected daily in each III 10
treatment for determination of dry weight. Shrimp were washed with distilled water and
oven-dried at 608C for 24 h. Dry weights were measured using a CAHN model C-33
microbalance with 0.001 mg precision.