2.1.Substrate preparation
Gossypium hirsutum waste, Oryza sativa straw and
Milicia excelsa sawdust were soaked separately in
water for moisture absorption. The substrates were
mixed evenly with rice bran supplement at different
concentration (20%, 15%, 10%, 5% and 0%
respectively. They were placed in transparent jam
bottles (250ml) in replicate of 3.