Measurement of serum vitamin D level [measured as 25-hydroxy cholecalciferol, 25(OH)D] was made in all subjects, using chemiluminescent microparticle immunoassay “ARCHITECT i1000SR-Abbott diagnostics; Abbott Laboratories, USA”. Normal level of vitamin D is defined as a 25-OH Vitamin D concentration greater than 30 ng/mL. Vitamin D insufficiency is defined as a 25-OH Vitamin D concentration of 20–30 ng/mL. Vitamin D deficiency is defined as a 25-OH Vitamin D level less than 20 ng/mL.
Serum level of Immunoglobulin E (total) was estimated using chemiluminescent microparticle immunoassay “ARCHITECT c4000 – Abbott diagnostics; Abbott Laboratories, USA” and expressed as IU/L. It was determined as elevation if serum T-IgE > 100 IU/L.
BMI is defined as body weight divided by the square of their height- with the value universally being given in units of kg/m2.