8) Each retained strain should be conserved through at least two independent clonal lineages.
Although the above recommendations are general, one should be careful not to generalize results obtained from a single field collection, as these may not be representative of the entire species.
For example, isolates from some wild specimens of A. subrufescens never produce fruiting bodies when domesticated; in A. bisporus isolates from field specimens generally fructify, but some with a very low yield and some have yields as great as the best cultivars (but not their level of quality).