Lipids yield can be increased by regulating the nutrients. The lipids yield is mainly affected by nitrogen. In diatoms, silicon affects the lipids yield. Other nutrients such as phosphate, sulfate, and iron also play crucial role in lipid accumulation [94]. The composition of lipids changes with nutrients. Microalage show high level of C16:0 and C18:0 in nutrients deprived condition. Yeesang et al. found relatively high level of lipid content at light intensity of 82 uE/m2/s and 0.74 mM of iron [79]. A variation in temperature can alter the lipid composition and its total yield. Un-saturated fatty acids show high yield at low temperature and saturated fatty at high temperature [94]. Light intensity also influences the composition of lipids. High light intensity helps to develop polar and neutral lipids, whereas polar lipids are produced at low light intensity. Light intensity changes the metabolism of fatty acid formation. High light intensity (>60 µmol/me/s) alter NADPH, pH and magnesium content of microalgae biomass. It is viewed that lipid content decrease at high light intensity. This decrease occurs due to inactivation or decrease in chloroplastidial activity at high light intensity [58] and [74]. Yoshioka et al. (2012) found an increase in lipid content of Isochrysis galbana by the intermittent supplement of blue light [95]. Microalgae accumulate more lipids under high light intensity. Jiang et al. found a biomass concentration of 2.23 g/L and lipid content of 59.9% under high light and nutrients-deprived condition [96].