Media preparation
The culture media described below were selected from literature
for selective enumeration of L. acidophilus in yoghurt and
cheese. The methodologies were strictly followed as described in
the references:Bile Agar MRS (MRSB)
Commercial MRS Agar (Difco e Becton, Dickinson and Company,
Franklin Lakes, USA) was prepared according to manufacturer's
instructions. The rehydrated medium was autoclaved at 121 C for
15 min. For bile solution, 10 g bile (B3883 e SigmaeAldrich Co., St.
Louis, USA) was dissolved in 100 mL distilled water, and the solution
was autoclaved at 121 C for 15 min. Immediately before use,
1.5 mL bile solution was added to 100 mL MRS agar