hows the change in Rg with freeze–thaw storage time. Only
the data before an elution time of 7.8 min are illustrated. This is because
the size of the fraction after an elution time of 7.8 min was not accurately
obtained, limited by the lower limit of the MALLS detector (approximately
10 nm). Fig. 5 shows that the Rg of the freeze–thaw stored
samples was lower than that of the control gluten. Additionally, the Rg
of the gluten samples decreased with freeze–thaw storage time. The
decrease in Rg is related to the breakage of disulphide bonds, which
results in the disruption of part of the branched chains and a decrease
in molecular weight