Figure 5. Histopathology of liver samples after treatment with different concentrations of
volatile oil from Blumea balsamifera (BB oil) in normal rats. (A) Group A1: Control (the
blank group) showed normal liver structure. (B) Group A2: Olive oil (vehicle) showed
diffused vacuolar changes. (C) Group A3: BB oil (100% w/v) 1000 mg/kg showed diffused
vacuolar changes; hepatocytes appeared pale with small discrete cytoplasmic vacuoles
(arrow). Hepatocytes showed clear cytoplasm and pyknotic nuclei (circles), suggesting
early mild hepatocellular necrosis. (D) Group A4: BB oil (50% w/v) 1000 mg/kg showed
diffused vacuolar changes; hepatocytes appeared pale with small discrete cytoplasmic
vacuoles or foamy cytoplasm (arrow); hepatocytes showed cytoplasm and pyknotic nuclei
(circles). However, cytoplasmic vacuoles and pyknotic nuclei were significant less than in
Group A3. (E) Group A5: BB oil (20% w/v) 1000 mg/kg showed diffused vacuolar changes;
hepatocytes appeared pale with small discrete cytoplasmic vacuoles (arrow).