Even though there are several reports available on micropropagation of Capsicum annum [4-6] and Capsicum frutescens [7, 8], research on micropropagation of Capsicum chinense is still in infant stage. Only few reports are available on the in vitro regeneration in Capsicum chinense [9-11] and this is the first report on successful plant regeneration from callus in Capsicum chinense Jacq.