Invasion and IF assay
When IEC monolayers were overlaid with the activated larvae
suspended in semisolid medium, the larvae browsed the IEC
surfaces, quickly invaded the monolayers and migrated through
contiguous cells (Figure 4A). The results of trypan blue staining
showed that the infective larvae caused a significant amount of
IEC damage. The mean area ([65612]6103
mm2
) of damage to
monolayer caused by activated larvae was significantly greater
than that (0.26103
mm2
) of damage to monolayer by non-
activated larvae (p,0.01).