Ethanol was produced from cassava starch by the
method of Anyakorah et al. (1998), as presented in
Figure 2. The steps involved in the production were:
Gelatinization (50 kg cassava starch was stirred in
200 ml water at 80oC until smooth gel was formed);
Liquefaction (4 ml of α-amylase was added to
gelatinized starch at 80oC and incubated for 60
min); Saccharification (4 ml of aqueous solution
of amylo-glucosidase was added to liquefied starch at 60oC and incubated for 240 min); Filtration (the
hydrolysate was filtered through muslin cloth);
Fermentation (yeast inoculum was added to the
filtrate before anaerobic incubation at 28oC) and
Distillation (fermentation broth was filtered and
the filtrate passed through the distillation unit twice
at 90oC).