Mentions: Asr expressing plants (green parts and tubers) did not exhibit any morphological changes in comparison to KD-UT plants (data not shown). In addition, starch morphology of Asr expressing plants was quite similar to that of KD-UT. With SEM, a rough surface was present on some of the (++) class transformant granules (Fig. 4B, F), but was considered as not significant when compared to dextran- (Fig. 4C, G) and mutan- (Fig. 4D, H) accumulating plants. In general, starch granules from the (+) and (−) class transformants were similar to those of the KD-UT (data not shown). Starch granules comparable to those illustrated in Fig. 4(F) were scored by analyzing a population of 100 granules in triplicate for each selected transformant (data not shown). KDA13, belonging to the (++) class transformant, exhibited (12% ± 1.0) of altered starch granules, followed by the (+) class transformant [KDA19 (9.3% ± 0.6); KDA27 (8.3% ± 0.6)]. For the (−) class transformant and KD-UT, the frequency of altered granules was lower, which was around the 7%.Fig. 4