In this method , spore of the fungus are suspended in a nonfat powdered mike solution or on sterile bovine serum , place in a small sterile tube, frozen rapidly in a dry ice bath , and dried under vacuum . The small pellet is sealed inside, under vacuum,by heatinf the ampule neck and is stored at refrigerator temperature .
Culture are revived by aseptically breaking open the ampule ,placing the pellet in sterile broth or distill water, and then streaking the rehydrate suspension onto suitable agar media .
In this method , spore of the fungus are suspended in a nonfat powdered mike solution or on sterile bovine serum , place in a small sterile tube, frozen rapidly in a dry ice bath , and dried under vacuum . The small pellet is sealed inside, under vacuum,by heatinf the ampule neck and is stored at refrigerator temperature .
Culture are revived by aseptically breaking open the ampule ,placing the pellet in sterile broth or distill water, and then streaking the rehydrate suspension onto suitable agar media .
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