2.4.3. Initial microbiological analysis (T0)
At T0 a WS was blended (Stomacher 400) for 60 s at room
temperature in 225 mL Buffered Peptone Water. Decimal dilutions
were carried out using the same diluent. TVC were obtained
on duplicate plates of Tryptone Glucose Yeast Agar
incubated at 32 ± 1 C for 48 h, S. aureus count was obtained on
Baird Parker agar incubated at 35 ± 1 C for 48 h and Listeria
strains were isolated by using Palcam Agar, incubated for 24 h at
37 ± 1 C. The suspected colonies of Listeria were characterized
by Gram stain. Gram positive colonies were tested for hemolysis
on Columbia Blood Agar and Bactident Catalase (Merk). Species
identification was made with API Listeria kit and Api Staph
(bioMerieux).