by mixing 7.5 ml of 12.5 mM
calcium chloride; 7.5 ml of 12.5 mM dibasic sodium
phosphate solution into 1.5 ml of M. elengi bark extract
solution (supernatant) by continuous stirring for
10–15 min. The mixture was sonicated (frequency 22 kHz,
power 120 W) for 30 min, dispersion was then filtered.