Currently, fish oil is the major commercial source of v-3 PUFA.
However, there are some limitations for fish oil use such as
peculiar taste, odor, stability problems, and resource sustainability
of fish stocks [13]. As marine algae is the primary
producer of v-3 PUFA in the natural environment, replacing fish
oil with v-3 PUFA enriched algae provides an excellent
opportunity to develop alternative v-3 PUFA [14]. The v-3
PUFA can be extracted from these algae biomass and used in
infant formulae, food, and pharmaceutical industry; or the algae
biomass can be used directly as a fish feed additive in the
aquaculture industry [15]. The alga Schizochytrium limacinum
has been reported as an excellentDHAproducer [16–19], aswell
as being capable of producing a high level ofDHAwhen growing
on glucose or glycerol [16]. When growing on glucose, its
zoospores were quickly induced and lipid bodies formed during
the synchronous growth [18]. However, growing the alga on
biodiesel-derived crude glycerol was not investigated. The
objective of thiswork was to investigate the potential of using this
alga to produce DHA from biodiesel industry-derived crude
glycerol.