This basic research was carried out on bio-ethanol derived from cellulosic waste (rice straw). In view of the fact that rice
straw is incorporated into the soil after harvesting the grains to increase mineral soil content, we examined the option of using
high pressure pretreatment which does not involve chemical treatment, thus allowing residues from bio-ethanol production to be
returned to the soil as a liquid fertilizer. Results from this study showed that i) high-pressure treatment enhanced the
saccharification of rice straw and ii) soil containing residues from bio-ethanol production did not inhibit the growth of Japanese
mustard spinach planted from seed