We applied RFLP analysis of ITS region for rapid identification of the yeast species
involved in spoilage of yoghurt. Some restriction
patterns generated from the region spanning the internal
transcribed spacers yielded a unique profile for
each species, and could be used as an easy and fast
method of routine yeast identification
We examined ten 125-g packs of retail yoghurt
showing evident signs of swelling. These samples
were taken from normal sales outlets and had not
reached their sell by date when the swelling occurred.
The types of product, the manufacturers and
the production dates were different.
The isolated strains did not ferment lactose and were positive for galactose fermentation, confirming the hypothesis
that galactose-fermenting yeast could be the cause of spoilage in yoghurt