Antioxidant capacity
The antioxidant capacity of samples was measured by applying
the procedure described previously (Arnao et al., 2001) with
little modifications. A 4.4 units/mL of peroxidase, 50 lM of
H2O2, 100 lM of 2, 2-azino-bis (3-ethylbenz-thiazoline-6-sulfonic
acid) and 1 mL distilled water were mixed and kept in
dark for 1 h for the reaction. After the addition of 1 mL of
plant material absorbance was determined at 734 nm. The
antioxidant capacity was calculated by the following formula