Yucatan pig’s porcine skin and intact subcutaneous tissue were harvested by using a 5×10 cm piece of the sample. The entire sample was then completely submerged in a CO2 incubator and allowed to equilibrate to 25oC. A patch-type electrode was attached to one side of the submerged porcine skin sample, and a 27-gauge RF needle electrode was inserted at 3 mm below the skin surface. The temperature change after RF treatment was measured with FLIR T400 (FLIR System Inc., Wilsonville, OR, USA).